产品描述
This Human HMOX2 ELISA Kit was designed for the quantitative measurement of Human HMOX2 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.
别名
Heme oxygenase (decycling) 2 ELISA Kit; Heme oxygenase (decyclizing) 2 ELISA Kit; Heme oxygenase 2 ELISA Kit; HMOX 2 ELISA Kit; Hmox2 ELISA Kit; HMOX2 protein ELISA Kit; HMOX2_HUMAN ELISA Kit; HO 2 ELISA Kit; HO-2 ELISA Kit; HO2 ELISA Kit; OTTHUMP00000159847 ELISA Kit
缩写
HMOX2
Uniprot No
P30519
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human HO-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 89 | | |
| Range % | 84-95 | | |
| 1:2 | Average % | 92 | | |
| Range % | 87-101 | | |
| 1:4 | Average % | 98 | | |
| Range % | 94-104 | | |
| 1:8 | Average % | 98 | | |
| Range % | 93-105 | | |
回收率
| The recovery of human HO-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 97 | 93-102 | | |
| EDTA plasma (n=4) | 91 | 84-97 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | | 20 | 2.447 | 2.292 | 2.370 | 2.222 | | | | 10 | 1.959 | 1.820 | 1.890 | 1.742 | | | | 5 | 1.161 | 1.251 | 1.206 | 1.058 | | | | 2.5 | 0.706 | 0.763 | 0.735 | 0.587 | | | | 1.25 | 0.406 | 0.416 | 0.411 | 0.263 | | | | 0.625 | 0.290 | 0.298 | 0.294 | 0.146 | | | | 0.312 | 0.221 | 0.223 | 0.222 | 0.074 | | | | 0 | 0.144 | 0.152 | 0.148 | | | |
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货期
3-5 working days