产品描述

The human IGF-1 ELISA kit is a solid-phase immunoassay specially designed to quantitatively measure human IGF-1 in serum, plasma, or tissue homogenates. It is based on the Sandwich-ELISA mechanism. IGF-1 in the sample is bound to the capture antibody immobilized on the 96-well strip plate and then sandwiched with the biotinylated IGF-1 antibody. After the addition of HRP-avidin and TMB substrate, the solution in the wells turns blue. The color reaction is stopped by adding the stop solution into the wells, and the color changes from blue to yellow. The color intensity is positively proportional to the IGF-1 bound in the initial step. The IGF-1 concentration can be calculated according to the standard curve. This kit is tested with high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.

IGF-1 plays a pivotal role in fetal development, adolescent growth, and adult tissue homeostasis. Together with IGF-2, IGF-1 is also important in skeletal muscle accrual during development, muscle maintenance, and aging, as well as during muscle injury. IGF-1 also participated in cellular differentiation and reproductive function. It is a survival agent and growth factor for a diverse range of normal and malignant cells. IGF-1 plays a key role in the anti-apoptotic and mitogenic pathways in many cell types. Altered IGF-1 has been linked to obesity and many related diseases. including cardiovascular diseases and cancer.

别名

IBP1 ELISA Kit; IGF I ELISA Kit; IGF IA ELISA Kit; IGF IB ELISA Kit; IGF-I ELISA Kit; Igf1 ELISA Kit; IGF1_HUMAN ELISA Kit; IGF1A ELISA Kit; IGFI ELISA Kit; IGFIA ELISA Kit; Insulin like growth factor 1 (somatomedin C) ELISA Kit; Insulin like growth factor 1 ELISA Kit; Insulin like growth factor IA ELISA Kit; Insulin like growth factor IB ELISA Kit; Insulin-like growth factor I ELISA Kit; Mechano growth factor ELISA Kit; MGF ELISA Kit; OTTHUMP00000195080 ELISA Kit; OTTHUMP00000195081 ELISA Kit; OTTHUMP00000195082 ELISA Kit; OTTHUMP00000195083 ELISA Kit; OTTHUMP00000195084 ELISA Kit; Somatomedia C ELISA Kit; Somatomedin C ELISA Kit; Somatomedin-C ELISA Kit

缩写

IGF-1

Uniprot No

P05019

灵敏度

1.95 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		96
		Range %		92-100
1:2		Average %		102
		Range %		99-105
1:4		Average %		93
		Range %		85-95
1:8		Average %		98
		Range %		94-101

回收率

The recovery of human IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		93		91-95
EDTA plasma (n=4)		96		92-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 500 2.785 2.752 2.769 2.627 250 2.219 2.189 2.204 2.062 125 1.388 1.298 1.343 1.201 62.5 0.702 0.724 0.713 0.571 31.2 0.434 0.446 0.440 0.298 15.6 0.307 0.315 0.311 0.169 7.8 0.228 0.216 0.222 0.080 0 0.143 0.141 0.142

货期

3-5 working days