产品描述
This Human DCD ELISA Kit was designed for the quantitative measurement of Human DCD protein in serum, plasma, cell culture supernates, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL .
别名
AIDD ELISA Kit; DCD 1 ELISA Kit; dcd ELISA Kit; DCD-1 ELISA Kit; DCD_HUMAN ELISA Kit; Dermcidin ELISA Kit; DSEP ELISA Kit; HCAP ELISA Kit; PIF ELISA Kit; Preproteolysin ELISA Kit
缩写
DCD
Uniprot No
P81605
灵敏度
0.156 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human PIF/DCD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:100 | Average % | 84 | |
| Range % | 80-92 | |
| 1:200 | Average % | 97 | |
| Range % | 91-105 | |
| 1:400 | Average % | 97 | |
| Range % | 92-110 | |
| 1:800 | Average % | 93 | |
| Range % | 86-98 | |
回收率
| The recovery of human PIF/DCD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 89-98 | |
| EDTA plasma (n=4) | 97 | 90-107 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 40 | 2.848 | 2.804 | 2.826 | 2.637 | | | 20 | 2.335 | 2.369 | 2.352 | 2.163 | | | 10 | 1.642 | 1.636 | 1.639 | 1.450 | | | 5 | 0.906 | 0.919 | 0.913 | 0.724 | | | 2.5 | 0.546 | 0.588 | 0.567 | 0.378 | | | 1.25 | 0.343 | 0.356 | 0.350 | 0.161 | | | 0.625 | 0.252 | 0.263 | 0.258 | 0.069 | | | 0 | 0.192 | 0.186 | 0.189 | | |
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货期
3-5 working days