产品描述
This Bovine IFN-tau ELISA Kit was designed for the quantitative measurement of Bovine IFN-tau protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.
别名
IFNT1 ELISA Kit; Interferon tau-1 ELISA Kit; IFN-tau-1 ELISA Kit; Antiluteolysin ELISA Kit; Trophoblast antiluteolytic protein ELISA Kit; Trophoblast protein 1 ELISA Kit; TP-1 ELISA Kit; Trophoblastin ELISA Kit
缩写
IFN-tau
Uniprot No
P15696
灵敏度
1.56 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine interferon-tau in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 85 | |
| Range % | 82-92 | |
| 1:2 | Average % | 98 | |
| Range % | 94-101 | |
| 1:4 | Average % | 92 | |
| Range % | 87-98 | |
| 1:8 | Average % | 95 | |
| Range % | 87-98 | |
回收率
| The recovery of bovine interferon-tau spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 90-96 | |
| EDTA plasma (n=4) | 96 | 91-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | 400 | 2.587 | 2.558 | 2.573 | 2.485 | | | 200 | 2.140 | 2.003 | 2.072 | 1.984 | | | 100 | 1.644 | 1.621 | 1.633 | 1.545 | | | 50 | 1.109 | 1.027 | 1.068 | 0.980 | | | 25 | 0.698 | 0.687 | 0.693 | 0.605 | | | 12.5 | 0.399 | 0.387 | 0.393 | 0.305 | | | 6.25 | 0.242 | 0.231 | 0.237 | 0.149 | | | 0 | 0.090 | 0.086 | 0.088 | | |
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货期
3-5 working days