产品描述
This Guinea pig IFN-γ ELISA Kit was designed for the quantitative measurement of Guinea pig IFN-γ protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.7 pg/mL .
别名
IFN-gamma,interferon gamma
缩写
IFN-γ
灵敏度
4.7 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 86 | |
| Range % | 81-92 | |
| 1:2 | Average % | 97 | |
| Range % | 92-103 | |
| 1:4 | Average % | 93 | |
| Range % | 88-108 | |
| 1:8 | Average % | 101 | |
| Range % | 96-105 | |
回收率
| The recovery of pig IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 97 | 93-102 | |
| EDTA plasma (n=4) | 90 | 86-95 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1200 | 2.075 | 2.051 | 2.063 | 1.965 | | | 600 | 1.465 | 1.441 | 1.453 | 1.355 | | | 300 | 1.069 | 1.045 | 1.057 | 0.959 | | | 150 | 0.655 | 0.622 | 0.639 | 0.541 | | | 75 | 0.400 | 0.387 | 0.394 | 0.296 | | | 37.5 | 0.320 | 0.295 | 0.308 | 0.210 | | | 18.75 | 0.205 | 0.223 | 0.214 | 0.116 | | | 0 | 0.101 | 0.095 | 0.098 | | |
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货期
3-5 working days