产品描述
This Human CYP3A4 ELISA Kit was designed for the quantitative measurement of Human CYP3A4 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.
别名
CYP3A4; CYP3A3; Cytochrome P450 3A4; 1,4-cineole 2-exo-monooxygenase; 1,8-cineole 2-exo-monooxygenase; Albendazole monooxygenase; sulfoxide-forming; Albendazole sulfoxidase; CYPIIIA3; CYPIIIA4; Cholesterol 25-hydroxylase; Cytochrome P450 3A3; Cytochrome P450 HLp; Cytochrome P450 NF-25; Cytochrome P450-PCN1; Nifedipine oxidase; Quinine 3-monooxygenase
缩写
CYP3A4
Uniprot No
P08684
灵敏度
5.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human CYP3A4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 90 | |
| Range % | 85-99 | |
| 1:2 | Average % | 96 | |
| Range % | 90-102 | |
| 1:4 | Average % | 97 | |
| Range % | 89-105 | |
| 1:5 | Average % | 95 | |
| Range % | 90-100 | |
回收率
| The recovery of human CYP3A4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 98 | 94-103 | |
| EDTA plasma (n=4) | 98 | 95-100 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1500 | 2.754 | 2.791 | 2.773 | 2.597 | | | 750 | 2.369 | 2.394 | 2.382 | 2.206 | | | 375 | 1.824 | 1.789 | 1.807 | 1.631 | | | 187.5 | 1.057 | 1.082 | 1.070 | 0.894 | | | 94 | 0.640 | 0.647 | 0.644 | 0.468 | | | 47 | 0.435 | 0.465 | 0.450 | 0.274 | | | 23.5 | 0.264 | 0.278 | 0.271 | 0.095 | | | 0 | 0.175 | 0.176 | 0.176 | | |
|
货期
3-5 working days