产品描述
This Human F-actin ELISA Kit was designed for the quantitative measurement of Human F-actin protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL .
缩写
F-actin
灵敏度
0.156 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human F-actin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 91 | |
| Range % | 86-95 | |
| 1:2 | Average % | 102 | |
| Range % | 97-107 | |
| 1:4 | Average % | 91 | |
| Range % | 85-97 | |
| 1:8 | Average % | 97 | |
| Range % | 91-103 | |
回收率
| The recovery of human F-actin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 91-101 | |
| EDTA plasma (n=4) | 88 | 85-96 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | 40 | 2.691 | 2.607 | 2.649 | 2.430 | | | 20 | 2.304 | 2.139 | 2.222 | 2.003 | | | 10 | 1.804 | 1.862 | 1.833 | 1.614 | | | 5 | 1.319 | 1.331 | 1.325 | 1.106 | | | 2.5 | 0.854 | 0.907 | 0.881 | 0.662 | | | 1.25 | 0.592 | 0.549 | 0.571 | 0.352 | | | 0.625 | 0.385 | 0.367 | 0.376 | 0.157 | | | 0 | 0.226 | 0.212 | 0.219 | | |
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货期
3-5 working days