产品描述
免疫球蛋白A(Immunoglobulin A,IgA)是免疫球蛋白家族的一种,是人体中分布最广泛的免疫球蛋白。它主要存在于体液中,如唾液、乳汁、泪液、鼻涕、肠液等,能够通过黏膜免疫屏障对机体进行保护。IgA具有免疫调节和免疫防御的作用,其水平变化与许多免疫相关的疾病,如感染、炎症、肿瘤等密切相关。
华美生物所提供的Human Immunoglobulin A,IgA ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆样本中的IgA,其灵敏度为0.24 ng/mL,检测范围为0.24 ng/mL-1000 ng/mL。
别名
N/A
缩写
IgA
灵敏度
0.24 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:10000 |
Average % |
89 |
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| Range % |
85-94 |
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| 1:20000 |
Average % |
90 |
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| Range % |
86-97 |
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| 1:40000 |
Average % |
96 |
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| Range % |
90-103 |
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| 1:80000 |
Average % |
89 |
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| Range % |
86-93 |
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回收率
| The recovery of human IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
91 |
89-94 |
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| EDTA plasma (n=4) |
92 |
88-97 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.180 |
2.205 |
2.193 |
2.124 |
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| 250 |
2.093 |
2.002 |
2.048 |
1.979 |
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| 62.5 |
1.841 |
1.868 |
1.855 |
1.786 |
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| 15.6 |
1.528 |
1.501 |
1.515 |
1.446 |
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| 3.9 |
0.772 |
0.753 |
0.763 |
0.694 |
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| 0.98 |
0.396 |
0.397 |
0.397 |
0.328 |
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| 0.24 |
0.189 |
0.180 |
0.185 |
0.116 |
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| 0 |
0.069 |
0.068 |
0.069 |
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货期
3-5 working days