产品描述
凋亡相关蛋白配体(Fas ligand,FasL)是Fas的配体,在免疫细胞、生殖细胞和肿瘤细胞等处表达。FasL与Fas的结合可以诱导细胞凋亡,参与细胞死亡、免疫耐受和免疫规避等生物学过程。
华美生物所提供的Human Factor-related Apoptosis ligand,FASL ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、细胞培养上清样本中的FASLG,其灵敏度为7.81 pg/mL,检测范围为31.25 pg/mL-2000 pg/mL。
别名
ALPS1B ELISA Kit; Apoptosis (APO 1) antigen ligand 1 ELISA Kit; Apoptosis antigen ligand 1 ELISA Kit; Apoptosis antigen ligand ELISA Kit; APT1LG1 ELISA Kit; APTL ELISA Kit; CD178 ELISA Kit; CD178 antigen ELISA Kit; CD95 ligand ELISA Kit; CD95-L ELISA Kit; CD95L ELISA Kit; CD95L protein ELISA Kit; Fas antigen ligand ELISA Kit; Fas L ELISA Kit; Fas ligand (TNF superfamily member 6) ELISA Kit; Fas ligand ELISA Kit; FASL ELISA Kit; Fasl Fas ligand (TNF superfamily member 6) ELISA Kit; FASLG ELISA Kit; Generalized lymphoproliferative disease ELISA Kit; Gld ELISA Kit; soluble form ELISA Kit; TNFL6_HUMAN ELISA Kit; TNFSF6 ELISA Kit; Tumor necrosis factor (ligand) superfamily member 6 ELISA Kit; Tumor necrosis factor ligand superfamily member 6 ELISA Kit
缩写
FASLG
Uniprot No
P48023
灵敏度
7.81 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human FASL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
89-98 |
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| 1:2 |
Average % |
98 |
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| Range % |
93-102 |
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| 1:4 |
Average % |
94 |
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| Range % |
89-97 |
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| 1:8 |
Average % |
92 |
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| Range % |
87-94 |
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回收率
| The recovery of human FASL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
89-97 |
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| EDTA plasma (n=4) |
88 |
82-94 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.472 |
2.624 |
2.548 |
2.423 |
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| 1000 |
1.568 |
1.467 |
1.518 |
1.393 |
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| 500 |
0.846 |
0.866 |
0.856 |
0.731 |
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| 250 |
0.488 |
0.474 |
0.481 |
0.356 |
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| 125 |
0.321 |
0.332 |
0.327 |
0.202 |
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| 62.5 |
0.255 |
0.267 |
0.261 |
0.136 |
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| 31.25 |
0.192 |
0.198 |
0.195 |
0.070 |
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| 0 |
0.124 |
0.126 |
0.125 |
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货期
3-5 working days