产品描述
This Pig IGF1 ELISA Kit was designed for the quantitative measurement of Pig IGF1 protein in serum, plasma, cell culture supernates, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 6.25 ng/mL-400 ng/mL and the sensitivity is 1.56 ng/mL .
别名
IGF1 ELISA Kit; Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit
缩写
IGF1
Uniprot No
P16545
灵敏度
1.56 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 85 | |
| Range % | 80-98 | |
| 1:2 | Average % | 95 | |
| Range % | 90-104 | |
| 1:4 | Average % | 97 | |
| Range % | 92-110 | |
| 1:8 | Average % | 95 | |
| Range % | 82-100 | |
回收率
| The recovery of pig IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 93 | 85-108 | |
| EDTA plasma (n=4) | 95 | 91-105 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 400 | 2.824 | 2.815 | 2.820 | 2.676 | | | 200 | 2.362 | 2.346 | 2.354 | 2.210 | | | 100 | 1.469 | 1.489 | 1.479 | 1.335 | | | 50 | 0.709 | 0.684 | 0.697 | 0.553 | | | 25 | 0.416 | 0.425 | 0.421 | 0.277 | | | 12.5 | 0.251 | 0.262 | 0.257 | 0.113 | | | 6.25 | 0.199 | 0.192 | 0.196 | 0.052 | | | 0 | 0.146 | 0.142 | 0.144 | | |
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货期
3-5 working days