产品描述
纤维连接蛋白1 (Fibronectin 1),是一种细胞外基质蛋白质,由FN1基因编码。FN1广泛存在于成纤维细胞、内皮细胞和其他细胞外基质组织中,并参与细胞粘附、细胞分化和细胞外基质组装等生理过程。在人类的体液和组织中,FN1的含量较高,尤其是在血浆和细胞外基质中。因此,测定FN1的含量可以用于监测组织纤维化、癌症进展和其他疾病的诊断。
华美生物所提供的Human Fibronectin,FN ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、组织匀浆、细胞培养上清、细胞裂解液样本中的FN1,其灵敏度为3.12 ng/mL,检测范围为12.5 ng/mL-800 ng/mL。
别名
CIG ELISA Kit; Cold insoluble globulin ELISA Kit; Cold-insoluble globulin ELISA Kit; DKFZp686F10164 ELISA Kit; DKFZp686H0342 ELISA Kit; DKFZp686I1370 ELISA Kit; DKFZp686O13149 ELISA Kit; ED B ELISA Kit; Fibronectin 1 ELISA Kit; FINC ELISA Kit; FINC_HUMAN ELISA Kit; FN ELISA Kit; FN1 ELISA Kit; FNZ ELISA Kit; GFND ELISA Kit; GFND2 ELISA Kit; LETS ELISA Kit; Migration stimulating factor ELISA Kit; MSF ELISA Kit; Ugl-Y3 ELISA Kit
缩写
FN1
Uniprot No
P02751
灵敏度
3.12 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human FN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
97 |
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| Range % |
92-104 |
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| 1:200 |
Average % |
95 |
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| Range % |
88-100 |
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| 1:400 |
Average % |
96 |
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| Range % |
89-103 |
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| 1:800 |
Average % |
93 |
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| Range % |
89-100 |
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回收率
| The recovery of human FN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-99 |
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| EDTA plasma (n=4) |
96 |
90-102 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 800 |
2.119 |
2.126 |
2.123 |
2.010 |
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| 400 |
1.797 |
1.704 |
1.751 |
1.638 |
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| 200 |
1.274 |
1.285 |
1.280 |
1.167 |
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| 100 |
0.758 |
0.761 |
0.760 |
0.647 |
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| 50 |
0.482 |
0.495 |
0.489 |
0.376 |
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| 25 |
0.286 |
0.296 |
0.291 |
0.178 |
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| 12.5 |
0.196 |
0.203 |
0.200 |
0.087 |
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| 0 |
0.112 |
0.113 |
0.113 |
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货期
3-5 working days