产品描述
This Mouse GSH-Px ELISA Kit was designed for the quantitative measurement of Mouse GSH-Px protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 U/mL-2000 U/mL and the sensitivity is 7.8 U/mL.
别名
Gpx1 ELISA Kit; Glutathione peroxidase 1 ELISA Kit; GPx-1 ELISA Kit; GSHPx-1 ELISA Kit; EC 1.11.1.9 ELISA Kit; Cellular glutathione peroxidase ELISA Kit; Selenium-dependent glutathione peroxidase 1 ELISA Kit
缩写
GSH-Px
Uniprot No
P11352
灵敏度
7.8 U/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse GSH-PX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 91 | | |
| Range % | 85-97 | | |
| 1:2 | Average % | 94 | | |
| Range % | 89-98 | | |
| 1:4 | Average % | 97 | | |
| Range % | 91-102 | | |
| 1:8 | Average % | 95 | | |
| Range % | 89-99 | | |
回收率
| The recovery of mouse GSH-PX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
|
| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 97 | 93-102 | | |
| EDTA plasma (n=4) | 88 | 82-94 | | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| U/ml | OD1 | OD2 | Average | Corrected | | | | 2000 | 2.324 | 2.401 | 2.363 | 2.275 | | | | 1000 | 1.602 | 1.699 | 1.651 | 1.563 | | | | 500 | 1.056 | 1.039 | 1.048 | 0.960 | | | | 250 | 0.657 | 0.665 | 0.661 | 0.573 | | | | 125 | 0.431 | 0.446 | 0.439 | 0.351 | | | | 62.5 | 0.274 | 0.285 | 0.280 | 0.192 | | | | 31.25 | 0.158 | 0.162 | 0.160 | 0.072 | | | | 0 | 0.087 | 0.088 | 0.088 | | | |
|
货期
3-5 working days