产品描述
This Human DEFB1 ELISA Kit was designed for the quantitative measurement of Human DEFB1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL .
别名
BD 1 ELISA Kit; BD-1 ELISA Kit; BD1 ELISA Kit; beta 1 ELISA Kit; Beta defensin 1 ELISA Kit; Beta-defensin 1 ELISA Kit; DEFB 1 ELISA Kit; DEFB1 ELISA Kit; DEFB1_HUMAN ELISA Kit; DEFB101 ELISA Kit; Defensin ELISA Kit; Defensin beta 1 ELISA Kit; Defensin beta 1 preproprotein ELISA Kit; HBD 1 ELISA Kit; hBD-1 ELISA Kit; HBD1 ELISA Kit; MGC51822 ELISA Kit
缩写
DEFB1
Uniprot No
P60022
灵敏度
0.78 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Microbiology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human β-defensins 1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:5 | Average % | 86 | |
| Range % | 80-90 | |
| 1:10 | Average % | 96 | |
| Range % | 91-100 | |
| 1:20 | Average % | 97 | |
| Range % | 91-105 | |
| 1:40 | Average % | 92 | |
| Range % | 86-98 | |
回收率
| The recovery of human β-defensins 1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 93 | 89-99 | |
| EDTA plasma (n=4) | 90 | 85-95 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 200 | 2.544 | 2.520 | 2.532 | 2.412 | | | 100 | 1.879 | 1.760 | 1.820 | 1.700 | | | 50 | 1.165 | 1.084 | 1.125 | 1.005 | | | 25 | 0.678 | 0.639 | 0.659 | 0.539 | | | 12.5 | 0.443 | 0.424 | 0.434 | 0.314 | | | 6.25 | 0.325 | 0.309 | 0.317 | 0.197 | | | 3.12 | 0.198 | 0.189 | 0.194 | 0.074 | | | 0 | 0.123 | 0.117 | 0.120 | | |
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货期
3-5 working days