产品描述
This rat DPP4 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of rat DPP4 in multiple samples, including serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the DPP4 levels in the sample and is measured at 450 nm through a microplate reader.
DPP4, also called CD26, is a serine protease that is widely distributed in various organs and cells. Through regulating energy metabolism, inflammation, and immune function, DPP4 is involved in various physiological and pathological processes such as inflammation, energy metabolism, immune regulation, cell adhesion, and apoptosis. During the COVID-19 global pandemic, DPP4 was found to be an important marker that may play a significant role in disease progression. DPP4 is also a surface marker expressed in senescent cells. DPP4 has also been demonstrated to be associated with CD8+ T cell co-stimulation, B cell activation, macrophage polarization, and NK cell proliferation, playing an emerging role in modulating immune functions. Regarding its role in the immune system, DPP4 was shown to participate in the pathogenesis of autoimmune diseases like rheumatoid arthritis, inflammatory bowel disease as well as graft-versus-host disease (GVHD).
别名
Dpp4; Cd26; Dipeptidyl peptidase 4; Bile canaliculus domain-specific membrane glycoprotein; Dipeptidyl peptidase IV; DPP IV; GP110 glycoprotein; T-cell activation antigen CD26; CD antigen CD26
缩写
DPP4
Uniprot No
P14740
灵敏度
0.195 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat DPPⅣin various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
94 |
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| Range % |
91-97 |
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| 1:2 |
Average % |
86 |
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| Range % |
83-90 |
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| 1:4 |
Average % |
101 |
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| Range % |
97-105 |
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| 1:8 |
Average % |
88 |
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| Range % |
85-93 |
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回收率
| The recovery of rat DPPⅣ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
106 |
103-109 |
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| EDTA plasma (n=4) |
95 |
91-99 |
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货期
3-5 working days
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