产品描述
This Bovine HSP70 ELISA Kit was designed for the quantitative measurement of Bovine HSP70 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 1.25 ng/mL-20 ng/mL and the sensitivity is 1.25 ng/mL.
缩写
HSP-70
Uniprot No
Q27975
灵敏度
1.25 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine HSP-70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
96 |
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| Range % |
91-101 |
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| 1:2 |
Average % |
90 |
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| Range % |
85-94 |
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| 1:4 |
Average % |
98 |
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| Range % |
92-103 |
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| 1:8 |
Average % |
93 |
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| Range % |
86-98 |
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回收率
| The recovery of bovine HSP-70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
84 |
80-89 |
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| EDTA plasma (n=4) |
95 |
91-99 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
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| 20 |
0.135 |
0.131 |
0.133 |
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| 10 |
0.219 |
0.231 |
0.225 |
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| 5 |
0.427 |
0.401 |
0.414 |
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| 2.5 |
0.747 |
0.792 |
0.770 |
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| 1.25 |
1.724 |
1.626 |
1.675 |
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货期
3-5 working days