产品描述

This Human IGF1R ELISA Kit was designed for the quantitative measurement of Human IGF1R protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

别名

CD221 ELISA Kit; CD221 antigen ELISA Kit; IGF 1 receptor ELISA Kit; IGF 1R ELISA Kit; IGF I receptor ELISA Kit; IGF-I receptor ELISA Kit; Igf1r ELISA Kit; IGF1R_HUMAN ELISA Kit; IGFIR ELISA Kit; IGFIRC ELISA Kit; IGFR ELISA Kit; Insulin like growth factor 1 receptor ELISA Kit; Insulin like growth factor 1 receptor precursor ELISA Kit; Insulin-like growth factor 1 receptor beta chain ELISA Kit; Insulin-like growth factor I receptor ELISA Kit; JTK13 ELISA Kit; MGC142170 ELISA Kit; MGC142172 ELISA Kit; MGC18216 ELISA Kit; Soluble IGF1R variant 1 ELISA Kit; Soluble IGF1R variant 2 ELISA Kit

缩写

IGF1R

Uniprot No

P08069

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human IGF-1R in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		91
		Range %		86-95
1:2		Average %		102
		Range %		97-107
1:4		Average %		91
		Range %		85-97
1:8		Average %		97
		Range %		91-103

回收率

The recovery of human IGF-1R spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		96		94-99
EDTA plasma (n=4)		95		89-99

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected   20 2.634 2.745 2.690 2.544   10 1.795 1.687 1.741 1.595   5 1.099 1.163 1.131 0.985   2.5 0.727 0.745 0.736 0.590   1.25 0.446 0.465 0.456 0.310   0.625 0.331 0.323 0.327 0.181   0.312 0.265 0.258 0.262 0.116   0 0.145 0.146 0.146

货期

3-5 working days