产品描述
This Mouse Ang1-7 ELISA Kit was designed for the quantitative measurement of Mouse Ang1-7 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.
缩写
Ang1-7
灵敏度
1.56 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Ang1-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 91 | |
| Range % | 86-95 | |
| 1:2 | Average % | 93 | |
| Range % | 87-99 | |
| 1:4 | Average % | 95 | |
| Range % | 89-102 | |
| 1:8 | Average % | 95 | |
| Range % | 87-98 | |
回收率
| The recovery of mouse Ang1-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 96 | 92-98 | |
| EDTA plasma (n=4) | 95 | 91-98 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 400 | 2.745 | 2.721 | 2.733 | 2.597 | | | 200 | 2.316 | 2.245 | 2.281 | 2.145 | | | 100 | 1.923 | 1.898 | 1.911 | 1.775 | | | 50 | 1.467 | 1.366 | 1.417 | 1.281 | | | 25 | 0.796 | 0.788 | 0.792 | 0.656 | | | 12.5 | 0.458 | 0.447 | 0.453 | 0.317 | | | 6.25 | 0.258 | 0.242 | 0.250 | 0.114 | | | 0 | 0.138 | 0.134 | 0.136 | | |
|
货期
3-5 working days