产品描述
This Dog Ang1-7 ELISA Kit was designed for the quantitative measurement of Dog Ang1-7 protein in serum, plasma, tissue homogenates, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.
缩写
Ang1-7
灵敏度
1.56 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of dog Ang1-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 87 | | |
| Range % | 83-92 | | |
| 1:2 | Average % | 100 | | |
| Range % | 97-103 | | |
| 1:4 | Average % | 86 | | |
| Range % | 83-89 | | |
| 1:8 | Average % | 98 | | |
| Range % | 94-102 | | |
回收率
| The recovery of dog Ang1-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 85 | 81-89 | | |
| EDTA plasma (n=4) | 91 | 85-94 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | | 400 | 3.061 | 2.900 | 2.981 | 2.871 | | | | 200 | 2.541 | 2.568 | 2.555 | 2.445 | | | | 100 | 1.751 | 1.768 | 1.760 | 1.650 | | | | 50 | 1.027 | 1.100 | 1.064 | 0.954 | | | | 25 | 0.505 | 0.549 | 0.527 | 0.417 | | | | 12.5 | 0.301 | 0.294 | 0.298 | 0.188 | | | | 6.25 | 0.198 | 0.189 | 0.194 | 0.084 | | | | 0 | 0.112 | 0.108 | 0.110 | | | |
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货期
3-5 working days