产品描述
The mouse angiopoietin-related protein 7 (ANGPTL7) ELISA Kit is used to quantitatively measure mouse ANGPTL7 concentrations in serum, plasma, or tissue homogenates. It performs well in important characteristics, including sensitivity and specificity. This assay is based on the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops proportionally to the amount of ANGPTL7 in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.
ANGPTL7, initially discovered in the stromal layer of the cornea, contributes to coordinating the trabecular meshwork's extracellular matrix and its response to steroids. Levels of ANGPTL7 has been shown to be increased in glaucoma and its overexpression elevates the collagen expression level. While ANGPTL7 induction by glucocorticoids led to the up-regulation of important glaucoma-related proteins including fibronectin, myocilin, and MMP1. ANGPTL7 overexpression has been detected in different human cancers. It exerts roles in various cancers through its interaction with the WNT/-beta-catenin signaling pathway.
别名
Angptl7Angiopoietin-related protein 7 ELISA kit; Angiopoietin-like protein 7 ELISA kit
缩写
ANGPTL7
Uniprot No
Q8R1Q3
灵敏度
1.56 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANGPTL7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 90 | |
| Range % | 86-94 | |
| 1:2 | Average % | 98 | |
| Range % | 93-103 | |
| 1:4 | Average % | 102 | |
| Range % | 95-109 | |
| 1:8 | Average % | 94 | |
| Range % | 90-98 | |
回收率
| The recovery of mouse ANGPTL7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 97 | 93-101 | |
| EDTA plasma (n=4) | 93 | 88-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 400 | 2.758 | 2.890 | 2.824 | 2.632 | | | 200 | 1.823 | 1.951 | 1.887 | 1.695 | | | 100 | 1.077 | 1.138 | 1.108 | 0.916 | | | 50 | 0.644 | 0.621 | 0.633 | 0.441 | | | 25 | 0.467 | 0.451 | 0.459 | 0.267 | | | 12.5 | 0.335 | 0.336 | 0.336 | 0.144 | | | 6.25 | 0.262 | 0.255 | 0.259 | 0.067 | | | 0 | 0.184 | 0.199 | 0.192 | | |
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货期
3-5 working days
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