产品描述
This Mouse CTX-Ⅱ ELISA Kit was designed for the quantitative measurement of Mouse CTX-Ⅱ protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL .
别名
C-telopeptide of type 2 collagen(CTX-2),_x000D_
C-telopeptide of type II collagen(CTX-II)
缩写
CTX-Ⅱ
灵敏度
0.78 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CTX-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:20 |
Average % |
93 |
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| Range % |
86-95 |
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| 1:40 |
Average % |
99 |
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| Range % |
96-102 |
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| 1:80 |
Average % |
92 |
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| Range % |
87-95 |
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| 1:160 |
Average % |
96 |
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| Range % |
93-99 |
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回收率
| The recovery of mouse CTX-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
89-96 |
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| EDTA plasma (n=4) |
94 |
91-96 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 200 |
2.290 |
2.256 |
2.273 |
2.112 |
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| 100 |
1.671 |
1.666 |
1.669 |
1.508 |
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| 50 |
1.143 |
1.132 |
1.138 |
0.977 |
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| 25 |
0.718 |
0.701 |
0.710 |
0.549 |
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| 12.5 |
0.412 |
0.427 |
0.420 |
0.259 |
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| 6.25 |
0.301 |
0.285 |
0.293 |
0.132 |
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| 3.12 |
0.228 |
0.221 |
0.225 |
0.064 |
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| 0 |
0.159 |
0.163 |
0.161 |
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货期
3-5 working days