产品描述
This Pig CST3 ELISA Kit was designed for the quantitative measurement of Pig CST3 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.78 μg/mL-50 μg/mL and the sensitivity is 0.2 μg/mL.
缩写
CST3
Uniprot No
Q0Z8R0
灵敏度
0.2 μg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV%<10% |
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig Cys-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
86 |
|
| Range % |
82-92 |
|
| 1:2 |
Average % |
93 |
|
| Range % |
89-97 |
|
| 1:4 |
Average % |
92 |
|
| Range % |
84-96 |
|
| 1:8 |
Average % |
95 |
|
| Range % |
90-100 |
|
回收率
| The recovery of pig Cys-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
97 |
9-103 |
|
| EDTA plasma (n=4) |
93 |
89-96 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| μg/ml |
OD1 |
OD2 |
Average |
|
|
| 50 |
0.202 |
0.205 |
0.204 |
|
|
| 25 |
0.305 |
0.307 |
0.306 |
|
|
| 12.5 |
0.446 |
0.431 |
0.439 |
|
|
| 6.25 |
0.646 |
0.658 |
0.652 |
|
|
| 3.12 |
0.851 |
0.863 |
0.857 |
|
|
| 1.56 |
1.582 |
1.497 |
1.540 |
|
|
| 0.78 |
2.045 |
1.973 |
2.009 |
|
|
| 0 |
2.758 |
2.604 |
2.681 |
|
|
|
货期
3-5 working days