产品描述
Aβ1-42是beta淀粉样蛋白(Aβ)的一种形式,它是一种由神经元合成和分泌的蛋白质。Aβ1-42是导致阿尔茨海默病(AD)的重要成分之一。它在AD病变过程中聚集成胶质斑和神经纤维缠结,损害神经元的正常功能。此外,Aβ1-42还可能在其他神经系统疾病的发病机制中发挥作用,包括创伤性脑损伤、脊髓灰质炎和肌萎缩侧索硬化症等。
华美生物所提供的Human amyloid beta peptide 1-42,Aβ1-42 ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、组织匀浆、脑脊液(CSF)样本中的Aβ1-42,其灵敏度为0.078 ng/mL,检测范围为0.312 ng/mL-20 ng/mL。
别名
Amyloid beta 42(ABeta 42),amyloid beta peptide 1-42,Aβ1-42,amyloid βpeptide 1-42
缩写
Aβ1-42
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human Aβ1-42 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
84 |
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| Range % |
80-92 |
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| 1:2 |
Average % |
97 |
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| Range % |
91-105 |
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| 1:4 |
Average % |
97 |
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| Range % |
92-110 |
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| 1:8 |
Average % |
93 |
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| Range % |
86-98 |
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回收率
| The recovery of human Aβ1-42 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-98 |
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| EDTA plasma (n=4) |
97 |
90-100 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.302 |
2.379 |
2.341 |
2.244 |
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| 10 |
1.762 |
1.781 |
1.772 |
1.675 |
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| 5 |
1.124 |
1.189 |
1.157 |
1.060 |
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| 2.5 |
0.653 |
0.679 |
0.666 |
0.569 |
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| 1.25 |
0.375 |
0.398 |
0.387 |
0.290 |
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| 0.625 |
0.251 |
0.259 |
0.255 |
0.158 |
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| 0.312 |
0.151 |
0.153 |
0.152 |
0.055 |
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| 0 |
0.095 |
0.098 |
0.097 |
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货期
3-5 working days