产品描述
This Human CDH2 ELISA Kit was designed for the quantitative measurement of Human CDH2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 ng/mL-400 ng/mL and the sensitivity is 1.56 ng/mL .
别名
CDH2; CDHN; NCAD; Cadherin-2; CDw325; Neural cadherin; N-cadherin; CD antigen CD325
缩写
CDH2
Uniprot No
P19022
灵敏度
1.56 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human N-Cad in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 88 | |
| Range % | 80-100 | |
| 1:2 | Average % | 97 | |
| Range % | 91-104 | |
| 1:4 | Average % | 97 | |
| Range % | 92-108 | |
| 1:8 | Average % | 94 | |
| Range % | 85-98 | |
回收率
| The recovery of human N-Cad spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 89-105 | |
| EDTA plasma (n=4) | 97 | 90-110 | |
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标准曲线
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml | OD1 | OD2 | Average | Corrected | |
| 400 | 2.152 | 2.174 | 2.163 | 2.005 | |
| 200 | 1.832 | 1.824 | 1.828 | 1.670 | |
| 100 | 1.220 | 1.251 | 1.236 | 1.078 | |
| 50 | 0.926 | 0.974 | 0.950 | 0.792 | |
| 25 | 0.574 | 0.543 | 0.559 | 0.401 | |
| 12.5 | 0.406 | 0.426 | 0.416 | 0.258 | |
| 6.25 | 0.335 | 0.312 | 0.324 | 0.166 | |
| 0 | 0.152 | 0.164 | 0.158 | | |
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货期
3-5 working days