产品描述
AXL(AXL receptor tyrosine kinase)是一种跨膜酪氨酸激酶受体,参与肿瘤、免疫调节、血管生成等生物学过程。其高表达与多种肿瘤的发生和进展相关,是潜在的治疗靶点。AXL的含量在不同组织和细胞类型中有所不同。
华美生物所提供的Human Tyrosine-protein kinase receptor UFO (AXL) ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人 血清、血浆、组织匀浆样本中的AXL,其灵敏度为7.81 pg/mL,检测范围为31.25 pg/mL-2000 pg/mL。
缩写
AXL
Uniprot No
P30530
灵敏度
7.81 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human AXL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
84-95 |
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| 1:2 |
Average % |
92 |
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| Range % |
85-99 |
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| 1:4 |
Average % |
90 |
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| Range % |
86-95 |
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| 1:8 |
Average % |
95 |
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| Range % |
85-100 |
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回收率
| The recovery of human AXL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
91-99 |
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| EDTA plasma (n=4) |
95 |
87-99 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.232 |
2.257 |
2.245 |
2.075 |
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| 1000 |
1.462 |
1.520 |
1.491 |
1.321 |
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| 500 |
0.859 |
0.871 |
0.865 |
0.695 |
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| 250 |
0.527 |
0.554 |
0.541 |
0.371 |
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| 125 |
0.325 |
0.335 |
0.330 |
0.160 |
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| 62.5 |
0.277 |
0.259 |
0.268 |
0.098 |
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| 31.25 |
0.221 |
0.233 |
0.227 |
0.057 |
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| 0 |
0.165 |
0.175 |
0.170 |
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货期
3-5 working days