产品描述
This Rabbit Col Ⅰ ELISA Kit was designed for the quantitative measurement of Rabbit Col Ⅰ protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.
别名
Collagen Type 1,Col 1,_x000D_
Collagen Type I,Col I
缩写
Col Ⅰ
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Col Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
85 |
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| Range % |
81-89 |
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| 1:2 |
Average % |
90 |
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| Range % |
85-96 |
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| 1:4 |
Average % |
88 |
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| Range % |
84-92 |
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| 1:8 |
Average % |
104 |
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| Range % |
100-109 |
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回收率
| The recovery of rabbit Col Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
90-96 |
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| EDTA plasma (n=4) |
99 |
94-103 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.013 |
2.136 |
2.075 |
1.935 |
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| 5 |
1.749 |
1.705 |
1.727 |
1.587 |
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| 2.5 |
1.388 |
1.498 |
1.443 |
1.303 |
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| 1.25 |
1.065 |
1.154 |
1.110 |
0.970 |
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| 0.625 |
0.754 |
0.788 |
0.771 |
0.631 |
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| 0.312 |
0.513 |
0.532 |
0.523 |
0.383 |
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| 0.156 |
0.312 |
0.331 |
0.322 |
0.182 |
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| 0 |
0.139 |
0.141 |
0.140 |
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货期
3-5 working days