产品描述
This Dog B2M ELISA Kit was designed for the quantitative measurement of Dog B2M protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 μg/mL-50 μg/mL and the sensitivity is 0.2 μg/mL.
别名
B2M ELISA Kit; Beta-2-microglobulin ELISA Kit; Fragment ELISA Kit
缩写
B2M
Uniprot No
P19341
灵敏度
0.2 μg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of dog β2-MG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 90 | | |
| Range % | 83-99 | | |
| 1:2 | Average % | 92 | | |
| Range % | 85-100 | | |
| 1:4 | Average % | 95 | | |
| Range % | 85-105 | | |
| 1:8 | Average % | 95 | | |
| Range % | 88-102 | | |
回收率
| The recovery of dog β2-MG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 95 | 86-99 | | |
| EDTA plasma (n=4) | 95 | 91-100 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| μg/ml | OD1 | OD2 | Average | Corrected | | | | 50 | 2.554 | 2.569 | 2.562 | 2.430 | | | | 25 | 2.272 | 2.256 | 2.264 | 2.132 | | | | 12.5 | 1.792 | 1.803 | 1.798 | 1.666 | | | | 6.25 | 1.153 | 1.180 | 1.167 | 1.035 | | | | 3.13 | 0.625 | 0.616 | 0.621 | 0.489 | | | | 1.56 | 0.363 | 0.379 | 0.371 | 0.239 | | | | 0.78 | 0.261 | 0.264 | 0.263 | 0.131 | | | | 0 | 0.135 | 0.129 | 0.132 | | | |
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货期
3-5 working days