产品描述
BAX (Bcl-2-associated X protein)是一种编码细胞凋亡的蛋白质,是Bcl-2家族的一个成员。它通过释放细胞质色素C促进凋亡。BAX是细胞凋亡通路中的关键分子,参与调控线粒体的透性,从而导致细胞凋亡。BAX在正常生理过程中也具有重要作用。它在正常细胞的有丝分裂中起到促进凋亡的作用,使得异常细胞更容易被身体识别和清除。
华美生物所提供的Rat Apoptosis regulator BAX(BAX) ELISA kit属于ELISA检测试剂盒,采用双抗夹心法定量检测大鼠血清、血浆、组织匀浆、细胞裂解液样本中的BAX,其灵敏度为15.6 pg/mL,检测范围为62.5 pg/mL-4000 pg/mL。
别名
Bax; Apoptosis regulator BAX
缩写
BAX
Uniprot No
Q63690
灵敏度
15.6 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat BAX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
89 |
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| Range % |
85-92 |
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| 1:2 |
Average % |
96 |
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| Range % |
91-105 |
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| 1:4 |
Average % |
90 |
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| Range % |
87-99 |
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| 1:8 |
Average % |
93 |
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| Range % |
82-98 |
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回收率
| The recovery of rat BAX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
88-97 |
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| EDTA plasma (n=4) |
97 |
93-105 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 4000 |
2.091 |
1.995 |
2.043 |
1.844 |
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| 2000 |
1.751 |
1.776 |
1.764 |
1.565 |
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| 1000 |
1.368 |
1.432 |
1.400 |
1.201 |
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| 500 |
0.985 |
0.972 |
0.979 |
0.780 |
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| 250 |
0.535 |
0.544 |
0.540 |
0.341 |
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| 125 |
0.348 |
0.352 |
0.350 |
0.151 |
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| 62.5 |
0.244 |
0.237 |
0.241 |
0.042 |
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| 0 |
0.201 |
0.197 |
0.199 |
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货期
3-5 working days