产品描述
This Rat CK-MM ELISA Kit was designed for the quantitative measurement of Rat CK-MM protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 μU/mL-500 μU/mL and the sensitivity is 1.95 μU/mL.
缩写
CK-MM
灵敏度
1.95 μU/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CK-MM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:100 | Average % | 105 | |
| Range % | 101-109 | |
| 1:200 | Average % | 87 | |
| Range % | 84-90 | |
| 1:400 | Average % | 102 | |
| Range % | 98-106 | |
| 1:800 | Average % | 105 | |
| Range % | 101-109 | |
回收率
| The recovery of rat CK-MM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 90-98 | |
| EDTA plasma (n=4) | 87 | 85-90 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| μU/ml | OD1 | OD2 | Average | | | | 500 | 0.080 | 0.078 | 0.079 | | | | 250 | 0.129 | 0.131 | 0.130 | | | | 125 | 0.201 | 0.215 | 0.208 | | | | 62.5 | 0.321 | 0.348 | 0.335 | | | | 31.25 | 0.542 | 0.501 | 0.522 | | | | 15.6 | 0.838 | 0.868 | 0.853 | | | | 7.8 | 1.168 | 1.198 | 1.183 | | | | 0 | 2.025 | 1.987 | 2.006 | | |
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货期
3-5 working days