产品描述
This Human AFP-L3 ELISA Kit was designed for the quantitative measurement of Human AFP-L3 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.3 ng/mL.
缩写
AFP-L3
灵敏度
0.3 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<10% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human AFP-L3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
84 |
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| Range % |
80-92 |
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| 1:2 |
Average % |
97 |
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| Range % |
91-105 |
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| 1:4 |
Average % |
97 |
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| Range % |
92-110 |
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| 1:8 |
Average % |
93 |
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| Range % |
86-98 |
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回收率
| The recovery of human AFP-L3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-98 |
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| EDTA plasma (n=4) |
97 |
90-104 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
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| 80 |
2.207 |
2.356 |
2.282 |
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| 40 |
1.205 |
1.213 |
1.209 |
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| 20 |
0.648 |
0.639 |
0.644 |
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| 10 |
0.318 |
0.324 |
0.321 |
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| 4 |
0.160 |
0.159 |
0.160 |
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| 2 |
0.098 |
0.095 |
0.097 |
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货期
3-5 working days