产品描述

This Human AKR1B10 ELISA Kit was designed for the quantitative measurement of Human AKR1B10 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL .

别名

AK1BA_HUMAN ELISA Kit; AKR1B10 ELISA Kit; AKR1B11 ELISA Kit; AKR1B12 ELISA Kit; Aldo keto reductase family 1 member B10 ELISA Kit; aldo keto reductase family 1 member B11 ELISA Kit; Aldo-keto reductase family 1 member B10 (aldose reductase) ELISA Kit; Aldo-keto reductase family 1 member B10 ELISA Kit; Aldo-keto reductase family 1 member B11 (aldose reductase-like) ELISA Kit; aldose reductase like 1 ELISA Kit; Aldose reductase like ELISA Kit; aldose reductase like peptide ELISA Kit; Aldose reductase related protein ELISA Kit; Aldose reductase-like ELISA Kit; Aldose reductase-related protein ELISA Kit; ALDRLn ELISA Kit; ARL 1 ELISA Kit; ARL-1 ELISA Kit; ARL1 ELISA Kit; ARL1 Human small intestine reductase ELISA Kit; ARP ELISA Kit; hARP ELISA Kit; HIS ELISA Kit; HSI ELISA Kit; SI reductase ELISA Kit; Small intestine reductase ELISA Kit

缩写

AKR1B10

Uniprot No

O60218

灵敏度

0.156 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Metabolism

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human AKR1B10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		90
		Range %		85-99
1:2		Average %		97
		Range %		91-100
1:4		Average %		89
		Range %		85-96
1:8		Average %		87
		Range %		83-92

回收率

The recovery of human AKR1B10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		93		89-97
EDTA plasma (n=4)		85		81-92

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 40 2.143 2.253 2.198 2.027 20 1.544 1.534 1.539 1.368 10 1.077 1.164 1.121 0.950 5 0.774 0.792 0.783 0.612 2.5 0.513 0.523 0.518 0.347 1.25 0.368 0.398 0.383 0.212 0.625 0.273 0.281 0.277 0.106 0 0.170 0.172 0.171

货期

3-5 working days