产品描述

This Human Bcl-xl ELISA Kit was designed for the quantitative measurement of Human Bcl-xl protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

别名

AI325008 ELISA Kit; BAD ELISA Kit; BAD_HUMAN ELISA Kit; BBC 2 ELISA Kit; BBC2 ELISA Kit; BBC6 ELISA Kit; Bcl 2 Antagonist of Cell Death ELISA Kit; Bcl 2 Binding Component 6 ELISA Kit; BCL X / BCL 2 Binding Protein ELISA Kit; BCL X Binding Protein ELISA Kit; Bcl XL/Bcl 2 Associated Death Promoter ELISA Kit; Bcl-2-binding component 6 ELISA Kit; Bcl-2-like protein 8 ELISA Kit; Bcl-XL/Bcl-2-associated death promoter ELISA Kit; Bcl2 antagonist of cell death ELISA Kit; BCL2 antagonist of cell death protein ELISA Kit; BCL2 associated agonist of cell death ELISA Kit; Bcl2 Associated Death Promoter ELISA Kit; BCL2 binding component 6 ELISA Kit; BCL2 binding protein ELISA Kit; Bcl2 Like 8 Protein ELISA Kit; Bcl2-L-8 ELISA Kit; BCL2L8 ELISA Kit; Proapoptotic BH3 Only Protein ELISA Kit

缩写

Bcl-xl

Uniprot No

Q92934

灵敏度

0.039 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human Bcl-xl in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		84
		Range %		80-89
1:2		Average %		97
		Range %		92-101
1:4		Average %		105
		Range %		100-110
1:8		Average %		93
		Range %		86-98

回收率

The recovery of human Bcl-xl spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		89-99
EDTA plasma (n=4)		90		85-94

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 10 2.415 2.352 2.384 2.190 5 1.934 1.876 1.905 1.711 2.5 1.405 1.504 1.455 1.261 1.25 0.902 0.939 0.921 0.727 0.625 0.544 0.524 0.534 0.340 0.312 0.378 0.359 0.369 0.175 0.156 0.291 0.309 0.300 0.106 0 0.191 0.197 0.194

货期

3-5 working days