产品描述

The human placenta lactogen (HPL) ELISA Kit is used to quantitatively measure human HPL concentrations in serum or plasma. It performs well in important characteristics, including sensitivity and specificity. This assay is based on the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops proportionally to the amount of HPL in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.

HPL (CSH1) is a peptide hormone secreted throughout pregnancy by syncytiotrophoblast cells in humans. It mainly functions to regulate maternal lipid and carbohydrate metabolism and is important for mother and fetus energy homeostasis. Indeed, HPL facilitates lipolysis of human adipose tissue in-vitro, increasing the circulating free fatty acids. These can be used as an energy source by producing ketone bodies, which act as fuel for the fetus. HPL also plays an important role in the regulation of insulin secretion in pancreatic β-cells, stimulating their proliferation, and promoting the expression of anti-apoptotic proteins. HPL mainly binds to prolactin receptors and with a much lower affinity to growth hormone receptors.

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These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 4000 2.028 2.067 2.048 2.002 1600 1.434 1.457 1.446 1.400 600 0.767 0.785 0.776 0.730 200 0.395 0.413 0.404 0.358 80 0.192 0.196 0.194 0.148 0 0.045 0.047 0.046

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