产品描述

The ELISA Kit is designed for quantitatively measuring human ANXA1 levels in samples, including serum, plasma, or tissue homogenates. It uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of ANXA1 in samples. The color intensity is measured at 450 nm via a microplate reader.

ANXA1 is a calcium-dependent phospholipid-binding protein with a phospholipase A2 inhibitory activity. ANXA1 exerts multiple roles in important biological processes such as cell differentiation, proliferation, plasma membrane repair, epithelial repair, and cell apoptosis. In normal health conditions, ANXA1 plays a major protective role in the brain through the promotion of blood-brain-barrier (BBB) integrity. Several studies have indicated a role for ANXA1 in neurological diseases, including Alzheimer’s disease (AD) and stroke. Studies have also shown that ANXA1 is involved in multiple anti-inflammatory processes, including regulation of neutrophil migration, macrophage phagocytosis, and induction of changes in cell polarity of microglial cells after ischemia-like injury in vitro.

别名

Annexin 1 ELISA Kit; Annexin A1 ELISA Kit; Annexin I (lipocortin I) ELISA Kit; Annexin I ELISA Kit; Annexin-1 ELISA Kit; AnnexinA1 ELISA Kit; AnnexinI ELISA Kit; ANX 1 ELISA Kit; ANX A1 ELISA Kit; ANX1 ELISA Kit; ANXA 1 ELISA Kit; ANXA1 ELISA Kit; ANXA1 protein ELISA Kit; ANXA1_HUMAN ELISA Kit; Calpactin 2 ELISA Kit; Calpactin II ELISA Kit; Calpactin-2 ELISA Kit; CalpactinII ELISA Kit; Chromobindin 9 ELISA Kit; Chromobindin-9 ELISA Kit; Chromobindin9 ELISA Kit; HGNC:533 ELISA Kit; Lipocortin 1 ELISA Kit; Lipocortin I ELISA Kit; Lipocortin1 ELISA Kit; LipocortinI ELISA Kit; LPC 1 ELISA Kit; LPC1 ELISA Kit; p35 ELISA Kit; Phospholipase A2 inhibitory protein ELISA Kit

缩写

ANXA1

Uniprot No

P04083

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ANX-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		85
		Range %		81-93
1:2		Average %		95
		Range %		90-100
1:4		Average %		98
		Range %		91-106
1:8		Average %		92
		Range %		80-98

回收率

The recovery of human ANX-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		80-110
EDTA plasma (n=4)		96		91-101

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected   20 1.958 1.872 1.915 1.772   10 1.794 1.696 1.745 1.602   5 1.405 1.384 1.395 1.252   2.5 0.887 0.839 0.863 0.720   1.25 0.534 0.524 0.529 0.386   0.625 0.342 0.319 0.331 0.188   0.312 0.236 0.219 0.228 0.085   0 0.149 0.137 0.143

货期

3-5 working days