产品描述

The product CSB-E11846h is a sandwich ELISA kit developed to measure concentrations of human CD44 in serum, plasma, cell culture supernates, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of CD44 in samples. The color intensity is measured at 450 nm via a microplate reader.

CD44 is a cell surface adhesion molecule overexpressed on cancer stem cells. Upon binding to its ligand hyaluronic acid (HA), CD44 is activated leading to the activation of cell signaling pathways that induce proliferation, increase cell survival, regulate cytoskeletal alterations, and enhance cellular motility. The interaction of CD44 with hyaluronan also contributes to tumor development, metastasis, and expression of the chemoresistant phenotype. In addition to hyaluronan uptake and degradation, CD44 also participates in angiogenesis, wound healing, as well as tissue formation and patterning. CD44 variants (CD44v) are up-regulated in neoplasia and involved in tumor metastasis and aggression.

别名

LHR ELISA Kit; BA-1 ELISA Kit; CD 44 ELISA Kit; CD44 ELISA Kit; CD44 antigen ELISA Kit; CD44 molecule (Indian blood group) ELISA Kit; CD44 molecule ELISA Kit; CD44_HUMAN ELISA Kit; CDw44 ELISA Kit; CDW44 antigen ELISA Kit; Cell surface glycoprotein CD44 ELISA Kit; chondroitin sulfate proteoglycan 8 ELISA Kit; CSPG8 ELISA Kit; ECMR-III ELISA Kit; Epican ELISA Kit; Extracellular matrix receptor III ELISA Kit; GP90 lymphocyte homing/adhesion receptor ELISA Kit; HCELL ELISA Kit; hematopoietic cell E- and L-selectin ligand ELISA Kit; Heparan sulfate proteoglycan ELISA Kit; Hermes antigen ELISA Kit; homing function and Indian blood group system ELISA Kit; HSA ELISA Kit; HUTCH-I ELISA Kit; HUTCH1 ELISA Kit; HUTCHI ELISA Kit; Hyaluronate receptor ELISA Kit; IN ELISA Kit; INLU-related p80 Glycoprotein ELISA Kit; MC56 ELISA Kit; MDU2 ELISA Kit; MDU3 ELISA Kit; MGC10468 ELISA Kit; MIC4 ELISA Kit; MUTCH I ELISA Kit; MUTCH1 ELISA Kit; PGP-1 ELISA Kit; PGP-I ELISA Kit; PGP1 ELISA Kit; Phagocytic glycoprotein 1 ELISA Kit; Phagocytic glycoprotein I ELISA Kit; Soluble CD44 ELISA Kit

缩写

CD44

Uniprot No

P16070

灵敏度

19.5 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human CD44 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:100		Average %		88
		Range %		80-92
1:200		Average %		98
		Range %		91-105
1:400		Average %		104
		Range %		92-110
1:800		Average %		94
		Range %		86-98

回收率

The recovery of human CD44 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		89-98
EDTA plasma (n=4)		96		90-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 5000 2.749 2.733 2.741 2.629 2500 1.823 1.801 1.812 1.700 1250 1.072 1.044 1.058 0.946 625 0.599 0.562 0.581 0.469 312 0.375 0.354 0.365 0.253 156 0.241 0.235 0.238 0.126 78 0.181 0.169 0.175 0.063 0 0.116 0.108 0.112

货期

3-5 working days