产品描述

The product CSB-E13423h is a sandwich ELISA kit developed to measure concentrations of human CD5L in serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of CD5L in samples. The color intensity is measured at 450 nm via a microplate reader.

CD5L is a secreted protein produced mostly by macrophages in lymphoid and inflamed tissues. It plays a critical role in immune homeostasis and inflammatory disease. It is involved in various diseases such as lipid metabolic disease, hepatocellular carcinoma, fungus-induced peritonitis, acute kidney injury, and myocardial infarction. CD5L localizes to CD36 to promote the transcription of genes involved in the regulation of mitochondrial biogenesis to maintain energy and metabolic homeostasis. CD5L also activates autophagy in macrophages while altering inflammatory phenotypes, specifically reducing TNF and IL-1 beta expression while elevating the immunosuppressive cytokine IL-10. Serum or plasma CD5L levels are increased in inflammatory conditions including liver cirrhosis, chronic hepatitis C, atopic dermatitis, and Kawasaki disease.

别名

AAC-11 ELISA Kit; AIM ELISA Kit; API6 ELISA Kit; APOPTOSIS INHIBITOR 6 ELISA Kit; APOPTOSIS INHIBITOR OF MACROPHAGES ELISA Kit; CD5 antigen like (scavenger receptor cysteine rich family) ELISA Kit; CD5 antigen-like ELISA Kit; Cd5l ELISA Kit; CD5L_HUMAN ELISA Kit; CT 2 ELISA Kit; CT-2 ELISA Kit; Highly similar to ANTIGEN WC1.1 [Bos taurus] ELISA Kit; IgM associated peptide ELISA Kit; IgM-associated peptide ELISA Kit; Pdp ELISA Kit; PRO229 ELISA Kit; SCAVENGER RECEPTOR CYSTEINE-RICH FAMILY ELISA Kit; SP ALPHA ELISA Kit; SP-alpha ELISA Kit; Spalpha ELISA Kit

缩写

CD5L

Uniprot No

O43866

灵敏度

1.56 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human CD5L in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		88
		Range %		84-92
1:2		Average %		103
		Range %		96-107
1:4		Average %		92
		Range %		87-94
1:8		Average %		101
		Range %		97-104

回收率

The recovery of human CD5L spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		97		93-101
EDTA plasma (n=4)		93		87-97

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 400 2.335 2.245 2.290 2.135 200 1.474 1.577 1.526 1.371 100 1.017 0.975 0.996 0.841 50 0.591 0.602 0.597 0.442 25 0.386 0.397 0.392 0.237 12.5 0.278 0.285 0.282 0.127 6.25 0.221 0.225 0.223 0.068 0 0.154 0.156 0.155

货期

3-5 working days