产品描述

This Human APOH ELISA Kit was designed for the quantitative measurement of Human APOH protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 ng/mL-1000 ng/mL and the sensitivity is 3.9 ng/mL.

别名

Activated protein C binding protein ELISA Kit; Activated protein C-binding protein ELISA Kit; Anticardiolipin cofactor ELISA Kit; APC inhibitor ELISA Kit; Apo-H ELISA Kit; APOH ELISA Kit; APOH_HUMAN ELISA Kit; apolipoprotein H (beta-2-glycoprotein I) ELISA Kit; Apolipoprotein H ELISA Kit; B2G1 ELISA Kit; B2GP1 ELISA Kit; B2GPI ELISA Kit; Beta 2 glycoprotein I ELISA Kit; Beta 2 glycoprotein I precursor ELISA Kit; Beta(2)GPI ELISA Kit; Beta-2-glycoprotein 1 ELISA Kit; Beta-2-glycoprotein I ELISA Kit; BG ELISA Kit; Glycoprotein 1; beta-2 ELISA Kit; Glycoprotein I; beta-2 ELISA Kit; OTTMUSP00000003033 ELISA Kit

缩写

APOH

Uniprot No

P02749

灵敏度

3.9 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human APOH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1000		Average %		91
		Range %		86-95
1:2000		Average %		102
		Range %		97-107
1:4000		Average %		91
		Range %		85-97
1:8000		Average %		97
		Range %		91-103

回收率

The recovery of human APOH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		89-98
EDTA plasma (n=4)		97		90-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected   1000 2.638 2.689 2.664 2.491   500 2.342 2.302 2.322 2.149   250 1.664 1.689 1.677 1.504   125 1.071 1.098 1.085 0.912   62.5 0.672 0.634 0.653 0.480   31.2 0.424 0.405 0.415 0.242   15.6 0.282 0.279 0.281 0.108   0 0.172 0.174 0.173

货期

3-5 working days