产品描述

This Human CTNNB1 ELISA Kit was designed for the quantitative measurement of Human CTNNB1 protein in serum, plasma, cell culture supernates, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

别名

b-catenin ELISA Kit; Beta catenin ELISA Kit; Beta-catenin ELISA Kit; Cadherin associated protein ELISA Kit; Catenin (cadherin associated protein); beta 1; 88kDa ELISA Kit; Catenin beta 1 ELISA Kit; Catenin beta-1 ELISA Kit; CATNB ELISA Kit; CHBCAT ELISA Kit; CTNB1_HUMAN ELISA Kit; CTNNB ELISA Kit; CTNNB1 ELISA Kit; DKFZp686D02253 ELISA Kit; FLJ25606 ELISA Kit; FLJ37923 ELISA Kit; OTTHUMP00000162082 ELISA Kit; OTTHUMP00000165222 ELISA Kit; OTTHUMP00000165223 ELISA Kit; OTTHUMP00000209288 ELISA Kit; OTTHUMP00000209289 ELISA Kit

缩写

CTNNB1

Uniprot No

P35222

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human CTNNB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		92
		Range %		87-98
1:2		Average %		93
		Range %		89-95
1:4		Average %		94
		Range %		90-98
1:8		Average %		96
		Range %		86-102

回收率

The recovery of human CTNNB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		97		92-104
EDTA plasma (n=4)		89		85-94

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1000 2.199 2.277 2.238 2.075 500 1.743 1.663 1.703 1.540 250 1.179 1.157 1.168 1.005 125 0.794 0.800 0.797 0.634 62.5 0.472 0.460 0.466 0.303 31.2 0.327 0.333 0.330 0.167 15.6 0.232 0.226 0.229 0.066 0 0.163 0.162 0.163

货期

3-5 working days