产品描述
CCL17蛋白是一种趋化因子,属于C-C趋化因子家族,也被称为TARC。它主要由树突状细胞、巨噬细胞、B细胞和其他免疫细胞分泌,并且能够吸引特定的免疫细胞进入炎症部位。
CCL17蛋白的生物学作用主要是通过结合其相应的受体CCR4来实现。CCR4广泛分布于多种免疫细胞表面,如T细胞、B细胞、树突状细胞等。它参与了多种炎症、免疫调节和过敏反应等生理过程。
CCL17蛋白在多种疾病的发生和发展中发挥着重要作用,如变态反应性疾病、自身免疫性疾病、肿瘤、感染性疾病等。因此,CCL17蛋白被认为是这些疾病的潜在治疗靶点。一些针对CCR4的药物已经在研发过程中,有望成为治疗相关疾病的新型治疗药物。
华美生物所提供的Human thymus activation regulated chemokine,TARC ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、组织匀浆样本中的CCL17,其灵敏度为3.9 pg/mL,检测范围为15.6 pg/mL-1000 pg/mL。
别名
A-152E5.3 ELISA Kit; A152E53 ELISA Kit; ABCD 2 ELISA Kit; ABCD2 ELISA Kit; C-C motif chemokine 17 ELISA Kit; CC chemokine TARC ELISA Kit; CCL17 ELISA Kit; CCL17_HUMAN ELISA Kit; Chemokine CC Motif Ligand 17 ELISA Kit; MGC138271 ELISA Kit; MGC138273 ELISA Kit; SCYA17 ELISA Kit; Small Inducible Cytokine A17 ELISA Kit; Small Inducible Cytokine A17 Precursor ELISA Kit; Small Inducible Cytokine Subfamily A (Cys Cys) ELISA Kit; Small Inducible Cytokine Subfamily A (Cys Cys) Member 17 ELISA Kit; Small-inducible cytokine A17 ELISA Kit; T Cell Directed CC Chemokine ELISA Kit; Thymus and activation regulated chemokine ELISA Kit; Thymus and activation-regulated chemokine ELISA Kit
缩写
CCL17
Uniprot No
Q92583
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human TARC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
106 |
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| Range % |
101-115 |
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| 1:10 |
Average % |
104 |
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| Range % |
102-108 |
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| 1:20 |
Average % |
87 |
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| Range % |
84-93 |
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| 1:40 |
Average % |
94 |
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| Range % |
90-97 |
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回收率
| The recovery of human TARC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
90-101 |
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| EDTA plasma (n=4) |
89 |
85-92 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.455 |
2.655 |
2.555 |
2.385 |
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| 500 |
2.015 |
2.101 |
2.058 |
1.888 |
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| 250 |
1.323 |
1.333 |
1.328 |
1.158 |
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| 125 |
0.738 |
0.802 |
0.770 |
0.600 |
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| 62.5 |
0.420 |
0.453 |
0.437 |
0.267 |
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| 31.2 |
0.288 |
0.282 |
0.285 |
0.115 |
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| 15.6 |
0.213 |
0.218 |
0.216 |
0.046 |
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| 0 |
0.169 |
0.171 |
0.170 |
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货期
3-5 working days