产品描述
This Rat 5-HIAA ELISA Kit was designed for the quantitative measurement of Rat 5-HIAA protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 25 ng/mL-400 ng/mL and the sensitivity is 25 ng/mL.
缩写
5-HIAA
灵敏度
25 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat 5-HIAA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
89-100 |
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| 1:2 |
Average % |
93 |
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| Range % |
88-97 |
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| 1:4 |
Average % |
98 |
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| Range % |
94-100 |
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| 1:8 |
Average % |
98 |
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| Range % |
94-102 |
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回收率
| The recovery of rat 5-HIAA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
87-96 |
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| EDTA plasma (n=4) |
94 |
91-98 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
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| 400 |
0.283 |
0.278 |
0.281 |
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| 200 |
0.540 |
0.527 |
0.534 |
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| 100 |
0.834 |
0.901 |
0.868 |
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| 50 |
1.698 |
1.676 |
1.687 |
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| 25 |
2.616 |
2.524 |
2.570 |
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货期
3-5 working days