产品描述
This Sheep 5HT/ST ELISA Kit was designed for the quantitative measurement of Sheep 5HT/ST protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 0.625 ng/mL-10 ng/mL and the sensitivity is 0.312 ng/mL .
缩写
5HT/ST
灵敏度
0.312 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Biochemicals
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of sheep 5HT/ST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:20 |
Average % |
84 |
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| Range % |
80-92 |
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| 1:40 |
Average % |
97 |
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| Range % |
91-105 |
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| 1:80 |
Average % |
96 |
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| Range % |
90-101 |
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| 1:160 |
Average % |
93 |
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| Range % |
86-98 |
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回收率
| The recovery of sheep 5HT/ST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-98 |
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| EDTA plasma (n=4) |
97 |
90-100 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
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| 10 |
0.122 |
0.115 |
0.119 |
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| 5 |
0.234 |
0.216 |
0.225 |
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| 2.5 |
0.404 |
0.407 |
0.406 |
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| 1.25 |
0.743 |
0.739 |
0.741 |
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| 0.625 |
1.221 |
1.194 |
1.208 |
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| 0 |
2.051 |
1.959 |
2.005 |
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货期
3-5 working days