产品描述
CLU蛋白是一种高度保守的糖蛋白,广泛存在于人体的多种组织和细胞中,包括肝脏、肾脏、肺、心脏、神经系统等。CLU蛋白的生物学功能非常复杂,参与了多种生理和病理过程。研究表明,CLU蛋白可以通过调节细胞凋亡、炎症、脂质代谢等多种途径参与心血管疾病、神经退行性疾病、糖尿病等多种疾病的发生和发展。此外,CLU蛋白还被认为是一种天然的神经保护因子,能够保护神经元免受氧化应激和神经炎症的损害。近年来,CLU蛋白在疾病的诊断和治疗方面受到了广泛关注,有望为治疗和预防多种疾病提供新的治疗策略和方法。
华美生物所提供的Human clusterin,CLU ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、细胞培养上清、唾液、尿液样本中的CLU,其灵敏度为0.78 ng/mL,检测范围为3.12 ng/mL-200 ng/mL。
别名
40 ELISA Kit; AAG 4 ELISA Kit; AAG4 ELISA Kit; Aging associated protein 4 ELISA Kit; Aging-associated gene 4 protein ELISA Kit; AI893575 ELISA Kit; APO J ELISA Kit; Apo-J ELISA Kit; APOJ ELISA Kit; ApoJalpha ELISA Kit; ApoJbeta ELISA Kit; Apolipoprotein J ELISA Kit; ApolipoproteinJ ELISA Kit; CLI ELISA Kit; CLU ELISA Kit; CLU1 ELISA Kit; CLU2 ELISA Kit; CLUS_HUMAN ELISA Kit; Clusterin alpha chain ELISA Kit; Clusterin ELISA Kit; Clusterin beta chain ELISA Kit; Complement associated protein SP 40 40 ELISA Kit; Complement associated protein SP 40 ELISA Kit; Complement associated protein SP40 ELISA Kit; Complement cytolysis inhibitor a chain ELISA Kit; Complement cytolysis inhibitor ELISA Kit; Complement cytolysis inhibitor b chain ELISA Kit; Complement lysis inhibitor ELISA Kit; Complement-associated protein SP-40 ELISA Kit; D14Ucla3 ELISA Kit; Dimeric acid glycoprotein ELISA Kit; Glycoprotein 80 ELISA Kit; Glycoprotein III ELISA Kit; GP80 ELISA Kit; Ku70-binding protein 1 ELISA Kit; KUB 1 ELISA Kit; KUB1 ELISA Kit; MGC24903 ELISA Kit; NA1/NA2 ELISA Kit; RATTRPM2B ELISA Kit; SGP 2 ELISA Kit; SGP2 ELISA Kit; SP 40 ELISA Kit; SP40 ELISA Kit; Sugp-2 ELISA Kit; Sulfated glycoprotein 2 ELISA Kit; Testosterone repressed prostate message 2 ELISA Kit; Testosterone-repressed prostate message 2 ELISA Kit; TRPM 2 ELISA Kit; TRPM-2 ELISA Kit; TRPM2 ELISA Kit; TRPM2B ELISA Kit; Trpmb ELISA Kit
缩写
CLU
Uniprot No
P10909
灵敏度
0.78 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human CLU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1000 |
Average % |
90 |
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| Range % |
85-96 |
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| 1:2000 |
Average % |
97 |
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| Range % |
91-104 |
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| 1:4000 |
Average % |
92 |
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| Range % |
85-100 |
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| 1:8000 |
Average % |
93 |
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| Range % |
83-99 |
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回收率
| The recovery of human CLU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
98 |
92-106 |
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| EDTA plasma (n=4) |
95 |
90-99 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 200 |
2.248 |
2.205 |
2.227 |
2.072 |
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| 100 |
1.196 |
1.266 |
1.231 |
1.076 |
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| 50 |
0.675 |
0.663 |
0.669 |
0.514 |
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| 25 |
0.472 |
0.453 |
0.463 |
0.308 |
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| 12.5 |
0.335 |
0.314 |
0.325 |
0.170 |
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| 6.25 |
0.221 |
0.207 |
0.214 |
0.059 |
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| 3.12 |
0.200 |
0.194 |
0.197 |
0.042 |
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| 0 |
0.155 |
0.154 |
0.155 |
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货期
3-5 working days