产品描述

The ELISA Kit is designed to measure human ANXA2 levels in samples, including serum, urine, saliva, or cell lysates. It uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of ANXA2 in samples. The color intensity is measured at 450 nm via a microplate reader.

ANXA2, a calcium-regulated phospholipid-binding protein, is involved in several critical cellular processes, such as cell proliferation, cell adhesion, cell motility, angiogenesis, vesicular transport, exocytosis, and endocytosis. It is abundantly expressed in multiple cancers, including breast cancer, squamous cervical cancer, pancreatic ductal adenocarcinoma (PDA), hepatocellular carcinoma (HCC), and lung cancer. ANXA2 plays a pivotal role in cancer cell proliferation, migration, invasion, metastasis, epithelial-mesenchymal transition (EMT), and treatment resistance. Abnormal expression of ANXA2 can be used as a potential predictive factor, diagnostic biomarker, and therapeutic target in cancer therapy.

别名

Annexin A2 ELISA Kit; Annexin II ELISA Kit; Annexin II; heavy chain ELISA Kit; Annexin-2 ELISA Kit; ANX 2 ELISA Kit; ANX2 ELISA Kit; ANX2L4 ELISA Kit; ANXA2 ELISA Kit; ANXA2_HUMAN ELISA Kit; arylsulfatase B ELISA Kit; CAL1H ELISA Kit; Calpactin I heavy chain ELISA Kit; calpactin I heavy polypeptide (p36) ELISA Kit; Calpactin I heavy polypeptide ELISA Kit; Calpactin-1 heavy chain ELISA Kit; chromobindin 8 ELISA Kit; Chromobindin-8 ELISA Kit; Epididymis secretory protein Li 270 ELISA Kit; HEL S 270 ELISA Kit; LIP2 ELISA Kit; Lipocortin II ELISA Kit; LPC2 ELISA Kit; LPC2D ELISA Kit; p36 ELISA Kit; P36 protein ELISA Kit; PAP-IV ELISA Kit; Placental anticoagulant protein IV ELISA Kit; Protein I ELISA Kit

缩写

ANXA2

Uniprot No

P07355

灵敏度

0.098 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ANX-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		90
		Range %		85-94
1:2		Average %		84
		Range %		80-88
1:4		Average %		97
		Range %		92-101
1:8		Average %		103
		Range %		96-110

回收率

The recovery of human ANX-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		90-99

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected   25 2.748 2.812 2.780 2.659   12.5 2.131 2.176 2.154 2.033   6.25 1.491 1.404 1.448 1.327   3.12 0.866 0.839 0.853 0.732   1.56 0.546 0.524 0.535 0.414   0.78 0.363 0.391 0.377 0.256   0.39 0.254 0.269 0.262 0.141   0 0.125 0.117 0.121

货期

3-5 working days