产品描述
cTn-I(心肌肌钙蛋白-I,cardiac Troponin-I)是一种心肌特异性蛋白,主要存在于心肌细胞中,是心肌收缩过程中的重要调节蛋白。cTn-I在心肌细胞中与其他肌钙蛋白一起组成肌钙蛋白复合体,调控心肌细胞的收缩和舒张过程。临床上,cTn-I常作为一种重要的生物标志物用于心肌损伤的诊断和评估,其含量的变化可以反映心肌损伤的程度和预后,对于急性心肌梗死等心血管疾病的诊断和治疗具有重要意义。
华美生物所提供的Rat Cardiac Troponin Ⅰ(cTn-Ⅰ) ELISA kit属于ELISA检测试剂盒,采用双抗夹心法定量检测大鼠血清、血浆、组织匀浆样本中的cTn-I,其灵敏度为7.81 pg/mL,检测范围为31.25 pg/mL-2000 pg/mL。
别名
Tnni3 ELISA Kit; Ctni ELISA Kit; Tni ELISA Kit; Troponin I ELISA Kit; cardiac muscle ELISA Kit; Cardiac troponin I ELISA Kit
缩写
cTn-Ⅰ
Uniprot No
P23693
灵敏度
7.81 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat cTn-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
88-98 |
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| 1:2 |
Average % |
98 |
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| Range % |
91-104 |
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| 1:4 |
Average % |
99 |
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| Range % |
94-105 |
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| 1:8 |
Average % |
87 |
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| Range % |
82-93 |
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回收率
| The recovery of rat cTn-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
88 |
82-92 |
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| EDTA plasma (n=4) |
95 |
90-100 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.321 |
2.220 |
2.271 |
2.118 |
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| 1000 |
1.340 |
1.441 |
1.391 |
1.238 |
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| 500 |
0.814 |
0.835 |
0.825 |
0.672 |
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| 250 |
0.529 |
0.540 |
0.535 |
0.382 |
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| 125 |
0.345 |
0.334 |
0.340 |
0.187 |
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| 62.5 |
0.261 |
0.250 |
0.256 |
0.103 |
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| 31.25 |
0.197 |
0.193 |
0.195 |
0.042 |
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| 0 |
0.153 |
0.152 |
0.153 |
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货期
3-5 working days