产品描述

This Human ATP1A1 ELISA Kit was designed for the quantitative measurement of Human ATP1A1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

别名

A1A1 ELISA Kit; AT1A1 ELISA Kit; AT1A1_HUMAN ELISA Kit; ATP1A1 ELISA Kit; Atpa-1 ELISA Kit; ATPase Na+/K+ transporting alpha 1 polypeptide ELISA Kit; ATPase Na+/K+ transporting subunit alpha 1 ELISA Kit; BC010319 ELISA Kit; EC 3.6.3.9 ELISA Kit; MGC3285 ELISA Kit; MGC38419 ELISA Kit; MGC51750 ELISA Kit; Na K ATPase alpha A catalytic polypeptide ELISA Kit; Na K ATPase catalytic subunit alpha A protein ELISA Kit; Na(+)/K(+) ATPase 1 ELISA Kit; Na(+)/K(+) ATPase alpha-1 subunit ELISA Kit; Na+; K+ ATPase alpha subunit ELISA Kit; Na+/K+ ATPase alpha 1 subunit ELISA Kit; Na+/K+ ATPase 1 ELISA Kit; Na,K ATPase alpha 1 subunit ELISA Kit; Nkaa1b ELISA Kit; Sodium potassium ATPase alpha 1 polypeptide ELISA Kit; Sodium pump 1 ELISA Kit; Sodium pump subunit alpha-1 ELISA Kit; sodium-potassium ATPase catalytic subunit alpha-1 ELISA Kit; Sodium/potassium-transporting ATPase subunit alpha-1 ELISA Kit

缩写

ATP1A1

Uniprot No

P05023

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ATP1A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		91
		Range %		80-100
1:2		Average %		96
		Range %		85-104
1:4		Average %		98
		Range %		92-104
1:8		Average %		96
		Range %		82-110

回收率

The recovery of human ATP1A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		94		84-106
EDTA plasma (n=4)		98		90-105

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1000 2.685 2.697 2.691 2.534 500 1.954 1.965 1.960 1.803 250 1.104 1.125 1.115 0.958 125 0.701 0.732 0.717 0.560 62.5 0.446 0.440 0.443 0.286 31.2 0.326 0.315 0.321 0.164 15.6 0.267 0.255 0.261 0.104 0 0.153 0.161 0.157

货期

3-5 working days