产品描述
This Mouse Ceruloplasmin (CP) ELISA Kit was designed for the quantitative measurement of Mouse Ceruloplasmin (CP) protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Competitive ELISA kit, its detection range is 31.2 ng/mL-2000 ng/mL and the sensitivity is 7.8 ng/mL.
别名
Cp ELISA Kit; Ceruloplasmin ELISA Kit; EC 1.16.3.1 ELISA Kit; Ferroxidase ELISA Kit
缩写
CP
Uniprot No
Q61147
灵敏度
7.8 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CP/CER in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1000 |
Average % |
95 |
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| Range % |
83-106 |
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| 1:2000 |
Average % |
92 |
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| Range % |
83-104 |
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| 1:4000 |
Average % |
95 |
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| Range % |
91-102 |
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| 1:8000 |
Average % |
93 |
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| Range % |
87-99 |
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回收率
| The recovery of mouse CP/CER spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
89-99 |
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| EDTA plasma (n=4) |
92 |
85-97 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
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| 2000 |
0.103 |
0.105 |
0.104 |
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| 1000 |
0.169 |
0.171 |
0.170 |
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| 500 |
0.301 |
0.309 |
0.305 |
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| 250 |
0.575 |
0.582 |
0.579 |
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| 125 |
1.294 |
1.306 |
1.300 |
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| 62.5 |
1.855 |
1.864 |
1.860 |
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| 31.2 |
2.536 |
2.541 |
2.539 |
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| 0 |
2.879 |
2.887 |
2.883 |
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货期
3-5 working days