产品描述
This Mouse Col Ⅱ ELISA Kit was designed for the quantitative measurement of Mouse Col Ⅱ protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.87 ng/mL-120 ng/mL and the sensitivity is 0.46 ng/mL.
别名
Collagen Type 2,Col 2,_x000D_
Collagen Type II,Col II
缩写
Col Ⅱ
灵敏度
0.46 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Col Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
81-92 |
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| 1:2 |
Average % |
101 |
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| Range % |
94-105 |
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| 1:4 |
Average % |
90 |
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| Range % |
85-94 |
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| 1:8 |
Average % |
97 |
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| Range % |
91-100 |
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回收率
| The recovery of mouse Col Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
89 |
82-93 |
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| EDTA plasma (n=4) |
104 |
95-108 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 120 |
2.668 |
2.511 |
2.590 |
2.401 |
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| 60 |
2.149 |
2.100 |
2.125 |
1.936 |
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| 30 |
1.459 |
1.445 |
1.452 |
1.263 |
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| 15 |
0.858 |
0.832 |
0.845 |
0.656 |
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| 7.5 |
0.540 |
0.555 |
0.548 |
0.359 |
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| 3.75 |
0.383 |
0.389 |
0.386 |
0.197 |
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| 1.87 |
0.292 |
0.298 |
0.295 |
0.106 |
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| 0 |
0.188 |
0.190 |
0.189 |
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货期
3-5 working days