产品描述
This Human CSN1S1 ELISA Kit was designed for the quantitative measurement of Human CSN1S1 protein in breast milk. It is a Competitive ELISA kit, its detection range is 37.5 μg/mL-600 μg/mL and the sensitivity is 18.75 μg/mL.
别名
Alpha casein ELISA Kit; alpha-S1-casein ELISA Kit; CASA ELISA Kit; CASA1_HUMAN ELISA Kit; Casein alpha s1 ELISA Kit; casein; alpha ELISA Kit; casein; alpha-S1; included ELISA Kit; Casoxin-D ELISA Kit; CSN1 ELISA Kit; CSN1S1 ELISA Kit; Csna ELISA Kit; MGC149368 ELISA Kit
缩写
CSN1S1
Uniprot No
P47710
灵敏度
18.75 μg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human CSN1S1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Breast milk(n=4) | |
| 1:10 | Average % | 90 | |
| Range % | 86-97 | |
| 1:20 | Average % | 93 | |
| Range % | 89-99 | |
| 1:40 | Average % | 95 | |
| Range % | 90-107 | |
| 1:80 | Average % | 92 | |
| Range % | 87-103 | |
回收率
| The recovery of human CSN1S1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Breast milk (n=5) | 95 | 87-101 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| μg/ml | OD1 | OD2 | Average | | | | 600 | 0.215 | 0.233 | 0.224 | | | | 300 | 0.349 | 0.374 | 0.362 | | | | 150 | 0.655 | 0.612 | 0.634 | | | | 75 | 1.358 | 1.326 | 1.342 | | | | 37.5 | 1.764 | 1.727 | 1.746 | | | | 0 | 2.631 | 2.419 | 2.525 | | |
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货期
3-5 working days