产品描述
This Rat BMP4 ELISA Kit was designed for the quantitative measurement of Rat BMP4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.
别名
Bmp4 ELISA Kit; Bmp-4 ELISA Kit; Dvr-4Bone morphogenetic protein 4 ELISA Kit; BMP-4 ELISA Kit; Bone morphogenetic protein 2B ELISA Kit; BMP-2B ELISA Kit
缩写
BMP4
Uniprot No
Q06826
灵敏度
1.95 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat BMP-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 98 | |
| Range % | 94-105 | |
| 1:2 | Average % | 91 | |
| Range % | 85-97 | |
| 1:4 | Average % | 86 | |
| Range % | 82-90 | |
| 1:8 | Average % | 94 | |
| Range % | 90-100 | |
回收率
| The recovery of rat BMP-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 89 | 85-93 | |
| EDTA plasma (n=4) | 92 | 87-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 500 | 2.514 | 2.651 | 2.583 | 2.468 | | | 250 | 1.867 | 1.797 | 1.832 | 1.717 | | | 125 | 1.279 | 1.315 | 1.297 | 1.182 | | | 62.5 | 0.885 | 0.842 | 0.864 | 0.749 | | | 31.2 | 0.513 | 0.538 | 0.526 | 0.411 | | | 15.6 | 0.294 | 0.289 | 0.292 | 0.177 | | | 7.8 | 0.201 | 0.213 | 0.207 | 0.092 | | | 0 | 0.112 | 0.118 | 0.115 | | |
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货期
3-5 working days