产品描述
This Human AQP1 ELISA Kit was designed for the quantitative measurement of Human AQP1 protein in serum, plasma, cell culture supernates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .
别名
AQP1; CHIP28; Aquaporin-1; AQP-1; Aquaporin-CHIP; Urine water channel; Water channel protein for red blood cells and kidney proximal tubule
缩写
AQP1
Uniprot No
P29972
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human AQP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 97 | |
| Range % | 90-103 | |
| 1:2 | Average % | 94 | |
| Range % | 88-100 | |
| 1:4 | Average % | 90 | |
| Range % | 85-95 | |
| 1:8 | Average % | 93 | |
| Range % | 87-99 | |
回收率
| The recovery of human AQP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 89 | 82-95 | |
| EDTA plasma (n=4) | 98 | 95-102 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.070 | 2.055 | 2.063 | 1.955 | | | 500 | 1.650 | 1.592 | 1.621 | 1.513 | | | 250 | 1.286 | 1.278 | 1.282 | 1.174 | | | 125 | 0.887 | 0.925 | 0.906 | 0.798 | | | 62.5 | 0.521 | 0.563 | 0.542 | 0.434 | | | 31.2 | 0.362 | 0.345 | 0.354 | 0.246 | | | 15.6 | 0.245 | 0.235 | 0.240 | 0.132 | | | 0 | 0.112 | 0.103 | 0.108 | | |
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货期
3-5 working days