产品描述
The Bovine CCL2 ELISA Kit is a tool for the quantification of C-C motif chemokine 2 (CCL2) in bovine serum, plasma, and tissue homogenates. CCL2 is a protein that plays a critical role in immune responses, particularly in the recruitment of immune cells to sites of inflammation. This ELISA kit detects CCL2 with high sensitivity, with a detection range of 78 pg/mL to 5000 pg/mL and a sensitivity of 0.039 ng/mL.
The assay principle is a sandwich measurement, providing quantitative results that can be obtained in as little as 1-5 hours. The kit is optimized for use with a sample volume of 50-100ul, making it a cost-effective and efficient solution for researchers.
CCL2 has been shown to be a reliable biomarker of immune responses. and this kit provides researchers with a precise and reliable method to accurately measure CCL2 levels in bovine samples.
别名
CCL2 ELISA Kit; SCYA2C-C motif chemokine 2 ELISA Kit; Acidic seminal fluid protein ELISA Kit; Monocyte chemotactic protein 1A ELISA Kit; MCP-1 ELISA Kit; MCP-1A ELISA Kit; Small-inducible cytokine A2 ELISA Kit
缩写
CCL2
Uniprot No
P28291
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine RBP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 88 | | |
| Range % | 82-95 | | |
| 1:2 | Average % | 98 | | |
| Range % | 94-102 | | |
| 1:4 | Average % | 97 | | |
| Range % | 90-105 | | |
| 1:8 | Average % | 86 | | |
| Range % | 82-92 | | |
回收率
| The recovery of bovine RBP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
|
| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 90 | 88-96 | | |
| EDTA plasma (n=4) | 100 | 96-103 | | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | | 10 | 1.921 | 1.890 | 1.906 | 1.792 | | | | 5 | 1.703 | 1.749 | 1.726 | 1.612 | | | | 2.5 | 1.345 | 1.431 | 1.388 | 1.274 | | | | 1.25 | 1.023 | 1.075 | 1.049 | 0.935 | | | | 0.625 | 0.671 | 0.722 | 0.697 | 0.583 | | | | 0.312 | 0.429 | 0.440 | 0.435 | 0.321 | | | | 0.156 | 0.282 | 0.289 | 0.286 | 0.172 | | | | 0 | 0.111 | 0.116 | 0.114 | | | |
|
货期
3-5 working days
已关闭
固定模块
收藏
易享客服
